SEC11A Monoclonal antibody

SEC11A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B11G12

Cat no : 67379-1-Ig

Synonyms

1810012E07Rik, Endopeptidase SP18, SEC11 homolog A, SEC11 like protein 1, SEC11A, SEC11L1, sid2895, SPase 18 kDa subunit, SPC18, SPCS4A

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Validation Data Gallery

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  WB analysis using 67379-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67379-1-Ig (SEC11A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67379-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67379-1-Ig (SEC11A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67379-1-Ig (SEC11A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67379-1-Ig (SEC11A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67379-1-Ig targets SEC11A in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SEC11A fusion protein Ag6610
• Full Name: SEC11 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 17-21 kDa
• GenBank Accession Number: BC000359
• Gene Symbol: SEC11A
• Gene ID (NCBI): 23478
• RRID: AB_2882626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC11A(Signal peptidase complex catalytic subunit SEC11A) is also named as SEC11L1, SPC18, SPCS4A and belongs to the peptidase S26B family. It is a component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. SEC11A has some isoforms with the molecular mass of 17-21 kDa.