SEC11A Polyclonal antibody

• Featured Product
• KD/KO Validated

SEC11A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14753-1-AP

Synonyms

1810012E07Rik, Endopeptidase SP18, SEC11 homolog A, SEC11 like protein 1, SEC11A, SEC11L1, sid2895, SPase 18 kDa subunit, SPC18, SPCS4A

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Validation Data Gallery

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  WB analysis of mouse testis using 14753-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14753-1-AP (SEC11A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 14753-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 14753-1-AP (SEC11A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 14753-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14753-1-AP (SEC11A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 14753-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14753-1-AP (SEC11A antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, human colon tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

14753-1-AP targets SEC11A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC11A fusion protein Ag6500
• Full Name: SEC11 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 17-21 kDa
• GenBank Accession Number: BC000359
• Gene Symbol: SEC11A
• Gene ID (NCBI): 23478
• RRID: AB_2186230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC11A(Signal peptidase complex catalytic subunit SEC11A) is also named as SEC11L1, SPC18, SPCS4A and belongs to the peptidase S26B family. It is a component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. SEC11A has some isoforms with the molecular mass of 17-21 kDa.

Publications

Species	Application	Title
human	WB	Nature; A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.; Authors - Rong Zhang ; KO Validated
human	WB	Cell Res; Switching off IMMP2L signaling drives senescence via simultaneous metabolic alteration and blockage of cell death.; Authors - Lifeng Yuan
human	WB	Mol Cell; Cleavage of the pseudoprotease iRhom2 by the signal peptidase complex reveals an ER-to-nucleus signaling pathway; Authors - Iqbal Dulloo