SDCCAG10 Polyclonal antibody

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  WB analysis of HepG2 using 16502-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16502-1-AP (SDCCAG10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 16502-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 16502-1-AP (SDCCAG10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16502-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16502-1-AP (SDCCAG10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16502-1-AP

  IP result of anti-SDCCAG10 (IP:16502-1-AP, 4ug; Detection:16502-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IF Staining of HepG2 using 16502-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16502-1-AP (SDCCAG10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SDCCAG10 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 16502-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Probable inactive peptidyl-prolyl cis-trans isomerase CWC27 homolog, PPIase CWC27, NY CO 10, CWC27, Antigen NY-CO-10

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, human colon tissue
• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16502-1-AP targets SDCCAG10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SDCCAG10 fusion protein Ag9671
• Full Name: serologically defined colon cancer antigen 10
• Calculated Molecular Weight: 472aa,54 kDa; 291aa,33 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC012117
• Gene Symbol: SDCCAG10
• Gene ID (NCBI): 10283
• RRID: AB_1851625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6UX04
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.