SDC2 Monoclonal antibody

SDC2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

CloneNo.

3A7B2

Cat no : 67088-1-Ig

Synonyms

Fibroglycan, HSPG, HSPG1, SDC2, SYND2, syndecan 2

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Validation Data Gallery

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  WB analysis using 67088-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67088-1-Ig (SDC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67088-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67088-1-Ig (SDC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: L02 cells, HuH-7 cells, HepG2 cells, A549 cells, NCI-H1299 cells, Jurkat cells
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

67088-1-Ig targets SDC2 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SDC2 fusion protein Ag5739
• Full Name: syndecan 2
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 19 kDa, 22 and 48 kDa
• GenBank Accession Number: BC049836
• Gene Symbol: SDC2
• Gene ID (NCBI): 6383
• RRID: AB_2882395
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDC2, containing cell surface heparan sulphate proteoglycan, functions as an integral membrane protein and participates in cell proliferation, cell migration, cell adhesion and signal transduction. It has been reported that the expression level of SDC2 will be up-regulated under hypoxia condition which can induce increased release of cytokines and chemokines. Besides, methylated SDC2 can be used as a potential biomarker for early diagnosis of colon cancer. 67088-1-Ig antibody detects the 19 and 22 kDa SDC2 isoforms as well as the 48 kDa dimeric protein in SDS-PAGE.(PMID: 18803305, 23297089, 30022330, 29945573)

Publications

Species	Application	Title
pig	IF	Sci Bull (Beijing); Single-cell dynamics of liver development in postnatal pigs; Authors - Lin Rao
human	WB	Cell Oncol (Dordr); Circular RNA circNFATC3 acts as a miR-9-5p sponge to promote cervical cancer development by upregulating SDC2.; Authors - Ningye Ma
human	WB	J Cell Mol Med; miR-218 affects the ECM composition and cell biomechanical properties of glioblastoma cells.; Authors - Małgorzata Grabowska
human	IHC	Cancer Cell Int; Identification of an immune-related signature indicating the dedifferentiation of thyroid cells.; Authors - Xuemin Wang
human	WB	Cancer Cell Int; GNG5 is a novel oncogene associated with cell migration, proliferation, and poor prognosis in glioma.; Authors - Wang Zhang
human	WB	Heart Vessels; Downregulation of lncRNA SNHG16 inhibits vascular smooth muscle cell proliferation and migration in cerebral atherosclerosis by targeting the miR-30c-5p/SDC2 axis.; Authors - Sheng-Nan Yang