CD138/Syndecan-1 Monoclonal antibody
CD138/Syndecan-1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
WB, IHC, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
4H5H5
Cat no : 60185-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF7 cells |
| Positive IHC detected in | human tonsil tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
The immunogen of 60185-2-Ig is CD138/Syndecan-1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD138/Syndecan-1 fusion protein Ag0823 |
| Full Name | syndecan 1 |
| Calculated Molecular Weight | 32.5 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC008765 |
| Gene Symbol | CD138 |
| Gene ID (NCBI) | 6382 |
| RRID | AB_11183769 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD138/Syndecan-1 antibody 60185-2-Ig | Download protocol |
| IHC protocol for CD138/Syndecan-1 antibody 60185-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol The Glypican-1/HGF/C-Met and Glypican-1/VEGF/VEGFR2 Ternary Complexes Regulate Hair Follicle Angiogenesis. |
