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CD138/Syndecan-1 Monoclonal antibody

CD138/Syndecan-1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B6A2

Cat no : 60185-1-Ig

Synonyms

CD138, CD138/Syndecan-1, SDC, SDC1, SYND1, syndecan, syndecan 1

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using CD138/Syndecan-1 Monoclonal antibody (60185-1-Ig)

    WB analysis of BxPC-3 using 60185-1-Ig

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60185-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inBXPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60185-1-Ig targets CD138/Syndecan-1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD138/Syndecan-1 fusion protein Ag0823
Full Name syndecan 1
Calculated Molecular Weight 32.5 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC008765
Gene Symbol CD138
Gene ID (NCBI) 6382
RRIDAB_10859833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.

Protocols

Product Specific Protocols
WB protocol for CD138/Syndecan-1 antibody 60185-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols