SCRIB Polyclonal antibody

SCRIB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 27083-1-AP

Synonyms

Protein scribble homolog, Protein LAP4, LAP4, KIAA0147, hScrib

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Validation Data Gallery

• 

  WB analysis using 27083-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27083-1-AP (SCRIB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27083-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27083-1-AP (SCRIB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27083-1-AP targets SCRIB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCRIB fusion protein Ag25795
• Full Name: scribbled homolog (Drosophila)
• Calculated Molecular Weight: 1630 aa, 175 kDa
• Observed Molecular Weight: 260 kDa
• GenBank Accession Number: BC036905
• Gene Symbol: SCRIB
• Gene ID (NCBI): 23513
• RRID: AB_2880749
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCRIB encodes a large, 260-kDa cytoplasmic scaffolding protein that comprises a large leucine-rich repeat (LRR) region and 4 PDZ domains that regulate protein-protein interactions. In mammals, cell polarity is established and maintained by at least 3 protein modules (Scrib, Crumbs, and Par). The apical (Crumbs and Par) and basolateral (Scrib) modules function in a mutually antagonistic relationship to regulate various polarization processes such as apical-basal polarity, planar cell polarity, asymmetric cell division and migration. The loss of SCRIB in malignant tumors suggest that it has potential to be a tumor suppressor. Alteration of polarity through deletion, downregulation, overexpression, and mislocalization can induce structural and functional alteration of cells that might be related to tumorigenesis. Mislocalization of SCRIB is involved in epithelial-to-mesenchymal transition (EMT) by inducing loss of E-cadherin. Deregulated SCRIB expression induces tumorigenesis in breast and liver. (PMID: 28460446, PMID: 32564009)

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; MiR-9-5p Inhibits the MMP+-Induced Neuron Apoptosis through Regulating SCRIB/β-Catenin Signaling in Parkinson's Disease.; Authors - Zhenyong Xiao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (06-20-2024)

Excellent for detection of Scribble by WB in endothelial cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC