Validation Data Gallery
|Positive WB detected in||mouse brain tissue, HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, mouse heart tissue, mouse brain tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:300-1:1200|
|Sample-dependent, check data in validation data gallery|
12175-1-AP targets OXCT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||OXCT1 fusion protein Ag2818|
|Full Name||3-oxoacid CoA transferase 1|
|Calculated molecular weight||520 aa, 56 kDa|
|Observed molecular weight||56 kDa|
|GenBank accession number||BC009001|
|Gene ID (NCBI)||5019|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)
|Product Specific Protocols|
|WB protocol for OXCT1 antibody 12175-1-AP||Download protocol|
|IHC protocol for OXCT1 antibody 12175-1-AP||Download protocol|
|IF protocol for OXCT1 antibody 12175-1-AP||Download protocol|
|IP protocol for OXCT1 antibody 12175-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kerry (Verified Customer) (09-26-2019)
Too much background. We tried all the dilutions you recommended in your literature and then more outside of both ends of that range.