SCNN1A Polyclonal antibody

SCNN1A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28707-1-AP

Synonyms

ENaCa, Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Alpha-ENaC, Alpha NaCH

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Validation Data Gallery

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  WB analysis using 28707-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28707-1-AP (SCNN1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

28707-1-AP targets SCNN1A in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCNN1A fusion protein Ag30063
• Full Name: sodium channel, nonvoltage-gated 1 alpha
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC006526
• Gene Symbol: SCNN1A
• Gene ID (NCBI): 6337
• RRID: AB_2918193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amiloride-sensitive sodium channel subunit alpha (SCNN1A, also known as Alpha-ENaC and ENaCA) is essential for maintaining sodium homeostasis and is particularly important in the distal nephron and late distal convoluted tubule of the kidney, where aldosterone triggers ENaC activity (PMID: 18086683; 31654198). The ~97 kDa and ~75kDa bands represent the glycosylated and unglycosylated forms of SCNN1A (PMID: 19462466). It also migrated as intact (≈ 75 kDa) and furin-cleaved (≈ 50 kDa) in urinary extracellular vesicles (uEVs) (PMID: 31654198).

Publications

Species	Application	Title
human	IF	PLoS One; The developmental programme for genesis of the entire kidney is recapitulated in Wilms tumour.; Authors - Ryuji Fukuzawa