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  • Western Blot (WB) analysis of various lysates using SCN3B Polyclonal antibody (25907-1-AP)

    WB analysis using 25907-1-AP (same clone as 25907-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25907-1-AP (SCN3B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25907-1-PBS in a different storage buffer formulation.

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Product Information

25907-1-PBS targets SCN3B in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCN3B fusion protein Ag23201 Predict reactive species
Full Name sodium channel, voltage-gated, type III, beta
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC126265
Gene Symbol SCN3B
Gene ID (NCBI) 55800
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NY72
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The sodium channel is a multi-subunit protein complex composed of a single large α-subunit along with smaller additional β-subunits. There are at least three different β-subunit genes, SCN1b, SCN2b, and SCN3b, all of which are expressed widely in excitable tissues. The bands on Western blots for SCN3b (45-50 kDa) were significantly larger than the predicted molecular weight, which is consistent with the protein being glycosylated. The two bands on the Western blot could be due to different levels of glycosylation or alternative spliced isoforms. (PMID: 11744748)