SCML2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SCML2 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25544-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 25544-1-AP (same clone as 25544-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25544-1-AP (SCML2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 25544-1-AP (same clone as 25544-1-PBS)

  WB result of SCML2 antibody (25544-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCML2 transfected HEK-293 cells. This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 25544-1-AP (same clone as 25544-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25544-1-AP (SCML2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 25544-1-AP (same clone as 25544-1-PBS)

  IP result of anti-SCML2 (IP:25544-1-AP, 4ug; Detection:25544-1-AP 1:300) with HEK-293 cells lysate 2400ug. This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 25544-1-AP (same clone as 25544-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 25544-1-AP (SCML2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25544-1-PBS in a different storage buffer formulation.

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Product Information

25544-1-PBS targets SCML2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCML2 fusion protein Ag22271
• Full Name: sex comb on midleg-like 2 (Drosophila)
• Calculated Molecular Weight: 700 aa, 77 kDa
• Observed Molecular Weight: 70 kDa, 80 kDa
• GenBank Accession Number: BC064617
• Gene Symbol: SCML2
• Gene ID (NCBI): 10389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The human SCML2 gene, a mammalian homologue of the Drosophila PcG protein SCM, encodes two protein isoforms: SCML2A that is bound to chromatin and SCML2B that is predominantly nucleoplasmic (PMID: 24358021). Polycomb repressive complex-1 (PRC1) is essential for the epigenetic regulation of gene expression. SCML2 is a Polycomb-group protein that associates with PRC1. SCML2 participates in the epigenetic control of transcription directly and in cooperation with PRC1 (PMID: 24986859).