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  • Featured Product
  • KD/KO Validated

SCML2 Polyclonal antibody

SCML2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25544-1-AP

Synonyms

SCML2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCML2 Polyclonal antibody (25544-1-AP)

    WB analysis using 25544-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25544-1-AP (SCML2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SCML2 Polyclonal antibody (25544-1-AP)

    WB analysis of HEK-293 using 25544-1-AP

    WB result of SCML2 antibody (25544-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCML2 transfected HEK-293 cells.

  • Western Blot (WB) analysis of mouse testis tissue using SCML2 Polyclonal antibody (25544-1-AP)

    WB analysis of mouse testis using 25544-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25544-1-AP (SCML2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SCML2 Polyclonal antibody (25544-1-AP)

    IP experiment of HEK-293 using 25544-1-AP

    IP result of anti-SCML2 (IP:25544-1-AP, 4ug; Detection:25544-1-AP 1:300) with HEK-293 cells lysate 2400ug.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SCML2 Polyclonal antibody (25544-1-AP)

    IF Staining of HEK-293 using 25544-1-AP

    Immunofluorescent analysis of HEK-293 cells using 25544-1-AP (SCML2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue, K-562 cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25544-1-AP is SCML2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCML2 fusion protein Ag22271
Full Name sex comb on midleg-like 2 (Drosophila)
Calculated Molecular Weight 700 aa, 77 kDa
Observed Molecular Weight 70 kDa, 80 kDa
GenBank Accession NumberBC064617
Gene Symbol SCML2
Gene ID (NCBI) 10389
RRIDAB_2880127
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human SCML2 gene, a mammalian homologue of the Drosophila PcG protein SCM, encodes two protein isoforms: SCML2A that is bound to chromatin and SCML2B that is predominantly nucleoplasmic (PMID: 24358021). Polycomb repressive complex-1 (PRC1) is essential for the epigenetic regulation of gene expression. SCML2 is a Polycomb-group protein that associates with PRC1. SCML2 participates in the epigenetic control of transcription directly and in cooperation with PRC1 (PMID: 24986859).

Protocols

Product Specific Protocols
WB protocol for SCML2 antibody 25544-1-APDownload protocol
IF protocol for SCML2 antibody 25544-1-APDownload protocol
IP protocol for SCML2 antibody 25544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols