SCGF Monoclonal antibody, PBS Only

SCGF Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H11E4

Cat no : 60295-1-PBS

Synonyms

CLEC11A, CLECSF3, LSLCL, P47, SCGF, Stem cell growth factor

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Validation Data Gallery

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  WB analysis of human heart using 60295-1-Ig (same clone as 60295-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 60295-1-Ig (CLEC11A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60295-1-Ig (CLEC11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60295-1-Ig (CLEC11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

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  IF Staining of human lymphoma using 60295-1-Ig (same clone as 60295-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using SCGF antibody (60295-1-Ig, Clone: 4H11E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60295-1-Ig (same clone as 60295-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SCGF antibody (60295-1-Ig, Clone: 4H11E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60295-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60295-1-PBS targets SCGF in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SCGF fusion protein Ag21365
• Full Name: C-type lectin domain family 11, member A
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC005810
• Gene Symbol: SCGF
• Gene ID (NCBI): 6320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CLEC11A, also named as CLECSF3, LSLCL, SCGF and p47, stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. CLEC11A acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. It suppresses SCF-stimulated erythrocyte proliferation.