Uteroglobin/CC10 Polyclonal antibody

Uteroglobin/CC10 Polyclonal Antibody for IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

22

Reviews (1)

Cat no : 10490-1-AP

Synonyms

SCGB1A1, Uteroglobin, Club cells 10 kDa secretory protein, Club cell phospholipid-binding protein, CCSP

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Validation Data Gallery

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  IHC staining of human lung cancer using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse lung using 10490-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10490-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse lung using 10490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using Uteroglobin/CC10 antibody (10490-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A549 using 10490-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human Uteroglobin/CC10 (10490-1-AP) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IHC detected in: mouse lung tissue, human lung cancer tissue, rat lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse lung tissue
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 9 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• FC: See 3 publications below

Product Information

10490-1-AP targets Uteroglobin/CC10 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Uteroglobin/CC10 fusion protein Ag0758
• Full Name: secretoglobin, family 1A, member 1 (uteroglobin)
• Calculated Molecular Weight: 10 kDa
• GenBank Accession Number: BC004481
• Gene Symbol: Uteroglobin
• Gene ID (NCBI): 7356
• RRID: AB_2183285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.

Publications

Species	Application	Title
human	IHC	Nature; SARS-CoV-2 Omicron variant replication in human bronchus and lung ex vivo.; Authors - Kenrie P Y Hui
human	IHC	Lancet Respir Med; Tropism, replication competence, and innate immune responses of the coronavirus SARS-CoV-2 in human respiratory tract and conjunctiva: an analysis in ex-vivo and in-vitro cultures.; Authors - Kenrie P Y Hui
human	IHC	Theranostics; A SCID mouse-human lung xenograft model of SARS-CoV-2 infection.; Authors - Wenkun Fu
human	IF	PLoS Pathog; Lung adenocarcinoma originates from retrovirus infection of proliferating type 2 pneumocytes during pulmonary post-natal development or tissue repair.; Authors - Murgia Claudio C
human,mouse	WB,IF	Am J Respir Cell Mol Biol; DACH1 Attenuates Airway Inflammation in COPD by Activating NRF2 Signaling; Authors - Qian Huang
mouse	IHC	J Pathol; Overexpression of the SARS-CoV-2 receptor ACE2 is induced by cigarette smoke in bronchial and alveolar epithelia.; Authors - Aibin Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tessa (Verified Customer) (02-03-2019)

Used in 20pcw human fetal lung and got nice staining. Looks very specific with low background. Found that it did not require antigen retrieval in cryosections. In fact it turned out better in sections where 10mM Na Citrate buffer at pH6 was not used.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Fetal Lung

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