SCFD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SCFD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12569-1-AP

Synonyms

Sly1p, SLY1 homolog, Sec1 family domain-containing protein 1, KIAA0917, FKSG23

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Validation Data Gallery

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  WB analysis using 12569-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12569-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12569-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12569-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12569-1-AP

  IP result of anti-SCFD1 (IP:12569-1-AP, 3ug; Detection:12569-1-AP 1:500) with HeLa cells lysate 2150ug.

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  IHC staining of human endometrial cancer using 12569-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12569-1-AP (SCFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 12569-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12569-1-AP (SCFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 12569-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12569-1-AP (SCFD1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 12569-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12569-1-AP (SCFD1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293T cells, human kidney tissue, human liver tissue, HEK-293 cells, HeLa cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IP: See 1 publications below

Product Information

12569-1-AP targets SCFD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, bacteria
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCFD1 fusion protein Ag3234
• Full Name: sec1 family domain containing 1
• Calculated Molecular Weight: 642 aa, 72 kDa
• Observed Molecular Weight: 66-72 kDa
• GenBank Accession Number: BC017734
• Gene Symbol: SCFD1
• Gene ID (NCBI): 23256
• RRID: AB_2183266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCFD1, also named as C14orf163, KIAA0917, STXBP1L2, Sly1p and SLY1, belongs to the STXBP/unc-18/SEC1 family. It is a component of the syntaxin-5 SNARE complex which regulates the early secretory pathway of eukaryotic cells at the level of endoplasmic reticulum (ER) to Golgi transport.(PMID:21242315) SCFD1 plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. It is involved in vesicular transport between the endoplasmic reticulum and the Golgi.

Publications

Species	Application	Title
human	WB	EMBO J; Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing.; Authors - Laufman Orly O
human	WB,IP	Autophagy; Acetylation of SCFD1 regulates SNARE complex formation and autophagosome-lysosome fusion.; Authors - Hong Huang ; KD Validated
human	WB	J Cell Sci; Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.; Authors - Renna Maurizio M ; KD Validated
Bacteria	WB	Microb Pathog; Formononetin alleviates Streptococcus suis infection by targeting suilysin.; Authors - Guizhen Wang
	WB	J Appl Microbiol; Silibinin attenuates Streptococcus suis serotype 2 virulence by targeting suilysin.; Authors - Xue Shen
human	WB	Mol Biol Cell; Recruitment of PI4KIIIβ to the Golgi by ACBD3 is dependent on an upstream pathway of a SNARE complex and golgins; Authors - Danièle Stalder ; KO Validated