Rat SCF Recombinant antibody, PBS Only (Detector)

SCF Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240787H1

Cat no : 83707-2-PBS

Synonyms

Kitlg, Mgf, Kitl, KIT ligand, 240787H1

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Validation Data Gallery

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  WB analysis using 83707-2-RR (same clone as 83707-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83707-2-RR (SCF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83707-2-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-12 using 83707-2-RR (same clone as 83707-2-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 83707-2-RR (SCF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83707-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP00664-1

  Sandwich ELISA standard curve of MP00664-1, Rat SCF Recombinant Matched Antibody Pair - PBS only. 83707-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1092. 83707-2-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL

• 

  Sample test of MP00664-1

  Serum of eight rats was measured. The rat SCF concentration of detected samples was determined to be 191.0 pg/mL with a range of 14.0 - 481.6 pg/mL.

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  Cytometric bead array standard curve of MP00664-1

  Cytometric bead array standard curve of MP00664-1, RAT SCF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83707-1-PBS. Detection antibody: 83707-2-PBS. Standard: Eg1092. Range: 0.313-20 ng/mL

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  Affinity and Kinetic Characterization of 83707-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83707-2-RR against Rat SCF were performed. The affinity constant is 0.624 nM.

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Product Information

83707-2-PBS targets SCF as part of a matched antibody pair:

MP00664-1: 83707-1-PBS capture and 83707-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: KIT ligand
• Calculated Molecular Weight: 31kDa
• Observed Molecular Weight: 28 kDa, 45 kDa
• GenBank Accession Number: NM_021843.4
• Gene Symbol: Kitlg
• Gene ID (NCBI): 60427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis. SCF is a 35 to 45 kDa monomeric glycoprotein.