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SCF Recombinant antibody

SCF Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240787H1

Cat no : 83707-2-RR

Synonyms

Kitlg, Mgf, Kitl, KIT ligand, 240787H1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCF Recombinant antibody (83707-2-RR)

    WB analysis using 83707-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83707-2-RR (SCF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using SCF Recombinant antibody (83707-2-RR)

    WB analysis of PC-12 using 83707-2-RR

    PC-12 cells were subjected to SDS PAGE followed by western blot with 83707-2-RR (SCF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83707-2-RR

    Affinity and Kinetic Characterization of 83707-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83707-2-RR against Rat SCF were performed. The affinity constant is 0.624 nM.

Tested Applications

Positive WB detected inCOLO 320 cells, PC-12 cells, Neuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83707-2-RR is SCF Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name KIT ligand
Calculated Molecular Weight31kDa
Observed Molecular Weight 28 kDa, 45 kDa
GenBank Accession NumberNM_021843.4
Gene Symbol Kitlg
Gene ID (NCBI) 60427
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis. SCF is a 35 to 45 kDa monomeric glycoprotein.

Protocols

Product Specific Protocols
WB protocol for SCF antibody 83707-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols