SCEL Polyclonal antibody

SCEL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 11906-1-AP

Synonyms

Sciellin

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Validation Data Gallery

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  WB analysis of human saliva using 11906-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 11906-1-AP (SCEL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skin using 11906-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11906-1-AP (SCEL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11906-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11906-1-AP (SCEL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11906-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 11906-1-AP (SCEL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human renal cell carcinoma using 11906-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11906-1-AP (SCEL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 11906-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11906-1-AP (SCEL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11906-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 11906-1-AP (SCEL antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 11906-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SCEL Polyclonal antibody (11906-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human saliva tissue, A431 cells, human skin tissue, mouse skin tissue
• Positive IHC detected in: human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11906-1-AP targets SCEL in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCEL fusion protein Ag2578
• Full Name: sciellin
• Calculated Molecular Weight: 668 aa, 75 kDa
• Observed Molecular Weight: 70-78 kDa
• GenBank Accession Number: BC020726
• Gene Symbol: SCEL
• Gene ID (NCBI): 8796
• RRID: AB_2183262
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.