• Featured Product
  • KD/KO Validated

SCD Polyclonal antibody

SCD Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28678-1-AP

Synonyms

Acyl CoA desaturase, Delta(9) desaturase, FADS5, Fatty acid desaturase, MSTP008, SCD, SCD1, SCDOS, Stearoyl CoA desaturase



Tested Applications

Positive WB detected inA431 cells, A375 cells, A549 cells, HepG2 cells
Positive IHC detected inmouse liver tissue, human liver tissue, mouse brown adipose tissue, human colon cancer tissue, human ovary tumor tissue, human stomach cancer tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28678-1-AP targets SCD in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCD fusion protein Ag29156
Full Name stearoyl-CoA desaturase (delta-9-desaturase)
Calculated Molecular Weight 355 aa, 41 kDa
Observed Molecular Weight28-42 kDa
GenBank Accession NumberBC005807
Gene Symbol SCD
Gene ID (NCBI) 6319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).

Protocols

Product Specific Protocols
WB protocol for SCD antibody 28678-1-APDownload protocol
IHC protocol for SCD antibody 28678-1-APDownload protocol
IF protocol for SCD antibody 28678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Free Radic Biol Med

Zinc deficiency drives ferroptosis resistance by lactate production in esophageal squamous cell carcinoma

Authors - Peiyan Yang
humanWB

Cells

Targeting Wnt/β-Catenin Signaling Exacerbates Ferroptosis and Increases the Efficacy of Melanoma Immunotherapy via the Regulation of MITF

Authors - Hao Wang
human,mouseWB

Cancers (Basel)

CircZBTB46 Protects Acute Myeloid Leukemia Cells from Ferroptotic Cell Death by Upregulating SCD

Authors - Fei Long
humanWB

Biomed Pharmacother

The anti-fibrotic efficacy of adelmidrol depends on hepatic PPARγ levels

Authors - Huanyu Xiang
human,mouseWB

Int Immunopharmacol

E3 ubiquitin ligase Siah1 aggravates NAFLD through Scp2 ubiquitination

Authors - Zhu Zhu
mouseIF

Nat Commun

Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration

Authors - Yang Yang