SCAMP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SCAMP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 26888-1-AP

Synonyms

C1orf3, PROPIN1, SCAMP3

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Validation Data Gallery

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  WB analysis using 26888-1-AP

  Huh7 cell lysates were subjected to SDS PAGE followed by western blot with 26888-1-AP (SCAMP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 26888-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26888-1-AP (SCAMP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 26888-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26888-1-AP (SCAMP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 26888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 26888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 26888-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HuH-7 cells, A549 cells, K-562 cells
• Positive IHC detected in: mouse skeletal muscle tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

26888-1-AP targets SCAMP3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCAMP3 fusion protein Ag25256
• Full Name: secretory carrier membrane protein 3
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC000161
• Gene Symbol: SCAMP3
• Gene ID (NCBI): 10067
• RRID: AB_2810962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	Mol Cell; mTORC1 Activation Requires DRAM-1 by Facilitating Lysosomal Amino Acid Efflux.; Authors - Florian Beaumatin ; KD Validated
human	WB	Aging (Albany NY); Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma.; Authors - Feiyu Mao
human	WB	Sci Rep; MicroRNA-27a/b-3p and PPARG regulate SCAMP3 through a feed-forward loop during adipogenesis.; Authors - Agné Kulyté
human	WB,IHC	Onco Targets Ther; SCAMP3 Promotes Glioma Proliferation and Indicates Unfavorable Prognosis via Multiple Pathways.; Authors - Chunliu Li ; KD Validated
human	WB	Onco Targets Ther; Bioinformatics Analysis and RNA-Sequencing of SCAMP3 Expression and Correlated Gene Regulation in Hepatocellular Carcinoma.; Authors - Shan-Shan Han
human	IF	Int J Biol Macromol; Fungal antitumor protein D1 is internalized via endocytosis and inhibits non-small cell lung cancer proliferation through MAPK signaling pathway; Authors - Min-Hui Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (02-15-2024)

This antibody provided some convincing IF staining but detected many unspecific bands when western blotting HUVEC lysate.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1 in 200 IF 1 in 1000 WB
• Cell Tissue Type: HUVEC