SATB2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SATB2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, chip, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 21307-1-AP

Synonyms

DNA binding protein SATB2, KIAA1034, SATB homeobox 2, SATB2

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Validation Data Gallery

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  WB analysis using 21307-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21307-1-AP (SATB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21307-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21307-1-AP (SATB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21307-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21307-1-AP (SATB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: L02 cells, human brain tissue, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Product Information

The immunogen of 21307-1-AP is SATB2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SATB2 fusion protein Ag15920
• Full Name: SATB homeobox 2
• Calculated Molecular Weight: 733 aa, 83 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC098136
• Gene Symbol: SATB2
• Gene ID (NCBI): 23314
• RRID: AB_10733104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse,rat	WB,IF	Pharmacol Res; Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.; Authors - Jiale Cai
human	chip	Aging (Albany NY); SATB2-Nanog axis links age-related intrinsic changes of mesenchymal stem cells from craniofacial bone.; Authors - Peipei Zhou
human	WB	Cell Cycle; In vitro experimental study on the formation of microRNA-34a loaded exosomes and their inhibitory effect in oral squamous cell carcinoma.; Authors - Wei Deng
rat	WB,IHC	Bone; miR-383 negatively regulates osteoblastic differentiation of bone marrow mesenchymal stem cells in rats by targeting Satb2.; Authors - Jianfei Tang ; KD Validated
human	IHC	Cancer Cell Int; Identification of key genes involved in tumor immune cell infiltration and cetuximab resistance in colorectal cancer.; Authors - Li Liang
human	WB	Mol Carcinog; Benzo[a]pyrene diol epoxide-induced transformed cells identify the significance of hsa_circ_0051488, a ERCC1-derived circular RNA in pulmonary squamous cell carcinoma.; Authors - Mingyang Xiao