SATB1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SATB1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15400-1-AP

Synonyms

Special AT-rich sequence-binding protein 1, DNA-binding protein SATB1

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Validation Data Gallery

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  WB analysis of human brain using 15400-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15400-1-AP (SATB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15400-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15400-1-AP (SATB1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15400-1-AP

  IP result of anti-SATB1 (IP:15400-1-AP, 4ug; Detection:15400-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human oesophagus cancer using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 15400-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 15400-1-AP (SATB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue, HEK-293 cells, mouse brain tissue, mouse spleen tissue, rat brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human oesophagus cancer tissue, human urothelial carcinoma tissue, mouse spleen tissue, mouse thymus tissue, rat brain tissue, rat spleen tissue, rat thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

15400-1-AP targets SATB1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SATB1 fusion protein Ag7615
• Full Name: SATB homeobox 1
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 86-100 kDa
• GenBank Accession Number: BC001744
• Gene Symbol: SATB1
• Gene ID (NCBI): 6304
• RRID: AB_2184321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epigenetic modifications and dynamic changes in chromatin organization by organizer proteins have recently been shown to play an instrumental role in regulating cancer-promoting genes. Special AT-rich binding protein (SATB1) is a unique type of global regulator that integrates higher-order chromatin organization -withregulation of gene expression. [PMID:23076250,22998183,23121661] SATB1 is a T cell-enriched transcription factor and a chromatin organizer essential for controlling genes that participate in T-cell development and activation. It regulates gene expression by periodically anchoring matrix attachment regions to the nuclear matrix and directly recruiting chromatin-modifying factors. Depending on its posttranslational modifications, SATB1 activates or represses multiple genes.Its expression is regulated by interleukin-4 (IL4) during T helper-2(Th2) cell differentiation[PMID: 20522714]. The calculated molecular weight of SATB1 is 86 kDa, but modified SATB1 is about 100 kDa (PMID: 22879953).

Publications

Species	Application	Title
human	WB,IP	Cell Rep; SATB1 regulates 3D genome architecture in T cells by constraining chromatin interactions surrounding CTCF-binding sites; Authors - Bao Wang ; KO Validated
mouse,rat	WB	Pharmacol Res; Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.; Authors - Jiale Cai
mouse	WB	Mol Neurobiol; SATB1/SLC7A11/HO-1 Axis Ameliorates Ferroptosis in Neuron Cells After Ischemic Stroke by Danhong Injection; Authors - Sikai Zhan
human	WB	Eur Rev Med Pharmacol Sci; Effect of STAT3 mediated epigenetic regulation in pre-eclampsia: an analysis of trial data.; Authors - X-L Zhang
human	WB	Int J Med Sci; SATB1 Knockdown Inhibits Proliferation and Invasion and Decreases Chemoradiation Resistance in Nasopharyngeal Carcinoma Cells by Reversing EMT and Suppressing MMP-9.; Authors - Dongni Zhou ; KD Validated
human	WB	Int J Clin Exp Pathol; Silencing SATB1 influences cell invasion, migration, proliferation, and drug resistance in nasopharyngeal carcinoma.; Authors - Chun-Sheng Ye ; KD Validated