SAT2 Polyclonal antibody, PBS Only

SAT2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16246-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 16246-1-AP (same clone as 16246-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16246-1-AP (SAT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16246-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 16246-1-AP (same clone as 16246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16246-1-AP (SAT2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 16246-1-AP (same clone as 16246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16246-1-AP (SAT2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16246-1-PBS in a different storage buffer formulation.

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Product Information

16246-1-PBS targets SAT2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAT2 fusion protein Ag9179
• Full Name: spermidine/spermine N1-acetyltransferase family member 2
• Calculated Molecular Weight: 170 aa, 19 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC011751
• Gene Symbol: SAT2
• Gene ID (NCBI): 112483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Spermidine/spermine N1-acetyltransferase 2 (SAT2) is a short-lived polyamine catabolic enzyme inducible by polyamines and polyamine analogues. Human SAT2 contains the highly conserved acetyl-CoA-binding domain and displays coactivator function to enhance NF-κB-dependent transcription and enhances TNFα-induced NF-κB activity (PMID: 17875644). Human SAT1 and SAT2 proteins, which share 46% amino acid sequence identity and are clearly homologous, both interact with HIF-1α and both promote the ubiquitination and degradation of HIF-1α (PMID: 17011643).