SART3 Polyclonal antibody

SART3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 18025-1-AP

Synonyms

p110 nuclear RNA-binding protein, p110, P100, KIAA0156, hSART 3

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Validation Data Gallery

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  WB analysis of Jurkat using 18025-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18025-1-AP (SART3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18025-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18025-1-AP (SART3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18025-1-AP

  IP result of anti-SART3 (IP:18025-1-AP, 4ug; Detection:18025-1-AP 1:700) with HeLa cells lysate 2000ug.

• 

  IHC staining of human testis using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18025-1-AP (SART3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18025-1-AP (SART3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18025-1-AP (SART3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue, human brain tissue, human kidney tissue, human liver tissue, human lung cancer tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue, mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Product Information

18025-1-AP targets SART3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SART3 fusion protein Ag12590
• Full Name: squamous cell carcinoma antigen recognized by T cells 3
• Calculated Molecular Weight: 963 aa, 110 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC093784
• Gene Symbol: SART3
• Gene ID (NCBI): 9733
• RRID: AB_2254228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The process of mRNA splicing required assembly of pre-mRNA into a large, dynamic RNA-protein complex, in which 5 small nuclear RNAs and more than 50 protein components are incorporated into functional small nuclear ribonucleoproteins(snRNPs). SART3 interacts with U6 snRNA and functioned as a U6-specific recycling factor responsible for regeneration of base-paired U4/U6 snRNPs from post-spliceosomal free U4 and U6 snRNPs. Also it can interact and regulate Tat transactivation activity, and act as a cellular factor for HIV-1 gene expression and viral replication.

Publications

Species	Application	Title
human,mouse	IP	RNA; 7SL RNA in Vertebrate Red Blood Cells.; Authors - Gaëlle J S Talhouarne
human	WB,IP	J Biol Chem; Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.; Authors - Zhe Chen
		Mol Cell Biol; USP15 deubiquitinates TUT1 associated with RNA metabolism and maintains cerebellar homeostasis.; Authors - Jaehyun Kim
human	IHC	Aging (Albany NY); SART3, regulated by p53, is a biomarker for diagnosis, prognosis and immune infiltration in hepatocellular carcinoma; Authors - Jusen Nong