SART1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SART1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22675-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 22675-1-AP (same clone as 22675-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22675-1-AP (SART1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 22675-1-AP (same clone as 22675-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22675-1-AP (SART1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 22675-1-AP (same clone as 22675-1-PBS)

  IP result of anti-SART1 (IP:22675-1-AP, 4ug; Detection:22675-1-AP 1:6000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 22675-1-AP (same clone as 22675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22675-1-AP (SART1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 22675-1-AP (same clone as 22675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22675-1-AP (SART1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 22675-1-AP (same clone as 22675-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using SART1 antibody 22675-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 22675-1-PBS in a different storage buffer formulation.

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Product Information

22675-1-PBS targets SART1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SART1 fusion protein Ag18478
• Full Name: squamous cell carcinoma antigen recognized by T cells
• Calculated Molecular Weight: 800 aa, 90 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC001058
• Gene Symbol: SART1
• Gene ID (NCBI): 9092
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Squamous cell carcinoma antigen recognized by T cells (SART1), also known as U4/U6.U5 tri-snRNP-associated 110 kDa protein, is a ubiquitously expressed protein that is involved in mRNA splicing and the regulation of cell proliferation. It has been identified as regulator of c-FLIP and drug-induced activation of caspase 8. The SART1 gene encodes two proteins, the SART1(800) protein is located in the nucleus of the majority of proliferating cells, while the SART1(259) protein is located in the cytosol of epithelial cancers. Genetic variation in the SART1 gene may be associated with breast cancer development. This antibody recognizes the endogenous 800-amino acid SART1, which migrates with an apparent molecular mass of 110 kDa in SDS-PAGE. (PMID: 22027693; 9449708; 19377877; 11350945)