SARS-CoV-2 S protein (944-1214 aa) Monoclonal antibody

SARS-CoV-2 S protein (944-1214 aa) Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Virus

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H5E6

Cat no : 67794-1-Ig

Synonyms

S protein, SARS-CoV-2 Spike Glycoprotein, SARS-CoV-2 Spike Glycoprotein (944-1214 aa), spike, spike protein

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Validation Data Gallery

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  WB analysis of Recombinant protein using 67794-1-Ig

  Spike Glycoprotein (944-1214 aa) were subjected to SDS PAGE followed by western blot with 67794-1-lg (SARS-CoV-2 Spike Glycoprotein (944-1214 aa) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: ag30682

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67794-1-Ig targets SARS-CoV-2 S protein (944-1214 aa) in WB, ELISA applications and shows reactivity with Virus samples.

• Tested Reactivity: Virus
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SARS-CoV-2 S protein (944-1214 aa) fusion protein Ag30682
• Full Name: SARS-CoV-2 Spike Protein
• Calculated Molecular Weight: 141 kDa
• GenBank Accession Number: NC_045512
• Gene Symbol: SARS-COV-2
• Gene ID (NCBI): 43740568
• RRID: AB_2918558
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).