Virus SARS-CoV-2 S protein (944-1214 aa) Monoclonal antibody, PBS Only

SARS-CoV-2 S protein (944-1214 aa) Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Virus

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H5E6

Cat no : 67794-1-PBS

Synonyms

S protein, SARS-CoV-2 Spike Glycoprotein, SARS-CoV-2 Spike Glycoprotein (944-1214 aa), spike, spike protein

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Validation Data Gallery

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  WB analysis of Recombinant protein using 67794-1-Ig (same clone as 67794-1-PBS)

  Spike Glycoprotein (944-1214 aa) were subjected to SDS PAGE followed by western blot with 67794-1-lg (SARS-CoV-2 Spike Glycoprotein (944-1214 aa) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67794-1-PBS in a different storage buffer formulation.

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Product Information

67794-1-PBS targets SARS-CoV-2 S protein (944-1214 aa) in WB, Indirect ELISA applications and shows reactivity with Virus samples.

• Tested Reactivity: Virus
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SARS-CoV-2 S protein (944-1214 aa) fusion protein Ag30682
• Full Name: SARS-CoV-2 Spike Protein
• Calculated Molecular Weight: 141 kDa
• GenBank Accession Number: NC_045512
• Gene Symbol: SARS-COV-2
• Gene ID (NCBI): 43740568
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).