SAR1B Monoclonal antibody

SAR1B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B11A1

Cat no : 67765-1-Ig

Synonyms

ANDD, CMRD, GTBPB, GTP binding protein B, GTP binding protein SAR1b, SAR1 homolog B (S. cerevisiae), SAR1B, SARA2, SARB

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Validation Data Gallery

• 

  WB analysis using 67765-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67765-1-Ig (SAR1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67765-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67765-1-Ig (SAR1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67765-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67765-1-Ig (SAR1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 67765-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67765-1-Ig (SAR1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: pig liver tissue, HeLa cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue, rabbit liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67765-1-Ig targets SAR1B in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SAR1B fusion protein Ag7718
• Full Name: SAR1 homolog B (S. cerevisiae)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC002847
• Gene Symbol: SAR1B
• Gene ID (NCBI): 51128
• RRID: AB_2918532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAR1B, also named as SARA2, SARB and GTBPB, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1B is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex.