Validation Data Gallery
|Positive WB detected in||mouse liver tissue, mouse brain tissue, mouse skeletal muscle tissue, mouse small intestine tissue|
|Positive IHC detected in||human liver tissue, human skeletal muscle tissue, human small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
22292-1-AP targets SAR1B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||SAR1 homolog B (S. cerevisiae)|
|Calculated molecular weight||22 kDa|
|Observed molecular weight||25-27 kDa|
|GenBank accession number||NM_016103|
|Gene ID (NCBI)||51128|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SAR1B, also named as SARA2, SARB and GTBPB, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1B is activated by the guanine nucleotide exchange factor PREB. It is involved in the selection of the protein cargo and the assembly of the COPII coat complex. The antibody is specific to SAR1B.
SAR1B senses leucine levels to regulate mTORC1 signalling.
J Clin Invest
Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.
Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis
J Biol Chem
The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.
J Biol Chem
Small intestine but not liver lysophosphatidylcholine acyltransferase 3 (Lpcat3) deficiency has a dominant effect on plasma lipid metabolism.