SAMD9 Polyclonal antibody

• Featured Product
• KD/KO Validated

SAMD9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22786-1-AP

Synonyms

OEF2, OEF1, NFTC, KIAA2004, DRIF1

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Validation Data Gallery

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  WB analysis of HEK-293 using 22786-1-AP

  WB result of SAMD9 antibody (22786-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SAMD9 transfected HEK-293 cells.

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  WB analysis using 22786-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22786-1-AP (SAMD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22786-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22786-1-AP (SAMD9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 22786-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 22786-1-AP (SAMD9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22786-1-AP targets SAMD9 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAMD9 fusion protein Ag18787
• Full Name: sterile alpha motif domain containing 9
• Calculated Molecular Weight: 1589 aa, 184 kDa
• Observed Molecular Weight: 148 kDa
• GenBank Accession Number: BC132775
• Gene Symbol: SAMD9
• Gene ID (NCBI): 54809
• RRID: AB_2879168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sterile alpha motif domain-containing protein 9(SAMD9), aslo named as C7orf5, DRIF1, KIAA2004, OEF1, plays a role in growth factor signal transduction. SAMD9 is a ubiquitously expressed cytoplasmic protein encoded by two juxtaposed single-exon genes on chromosome 7q21(PMID: 34675305). Overexpression of SAMD9 protein in tissue culture results in reduction of cell proliferation rate and invasion index, and increasing activity of caspase 3(PMID: 25450373). An important paralog of this gene is SAMD9L. Observed MW of SAMD9 is from 148 kDa(PMID: 21248034).