SALL4 Monoclonal antibody

SALL4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2B5B2

Cat no : 66236-1-Ig

Synonyms

DRRS, HSAL4, sal like 4 (Drosophila), Sal like protein 4, SALL4, Zinc finger protein SALL4, ZNF797

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Validation Data Gallery

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  WB analysis of K-562 using 66236-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66236-1-Ig (SALL4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66236-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66236-1-Ig (SALL4 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

66236-1-Ig targets SALL4 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SALL4 fusion protein Ag17480
• Full Name: sal-like 4 (Drosophila)
• Calculated Molecular Weight: 1053 aa, 112 kDa
• Observed Molecular Weight: 165 kDa
• GenBank Accession Number: BC111714
• Gene Symbol: SALL4
• Gene ID (NCBI): 57167
• RRID: AB_2881625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SALL4, also named as Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta
-catenin and activate the Wnt/beta
-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids.

Publications

Species	Application	Title
human	WB	Cancer Chemother Pharmacol; Sorafenib combined with dasatinib therapy inhibits cell viability, migration, and angiogenesis synergistically in hepatocellular carcinoma.; Authors - Chiung-Chi Cheng
human	IHC	Pediatr Surg Int; SALL4 is a useful marker for pediatric yolk sac tumors.; Authors - Peng Wu