SAA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SAA1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16721-1-AP

Synonyms

Serum amyloid protein A(3-104), Serum amyloid protein A(2-104), Serum amyloid protein A(2-103), Serum amyloid protein A(2-102), Serum amyloid A-1 protein

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Validation Data Gallery

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  IHC staining of human liver using 16721-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16721-1-AP (SAA1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16721-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16721-1-AP (SAA1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16721-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16721-1-AP (SAA1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 16721-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16721-1-AP (SAA1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human liver cancer tissue, human liver tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

16721-1-AP targets SAA1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAA1 fusion protein Ag10160
• Full Name: serum amyloid A1
• Calculated Molecular Weight: 122 aa, 14 kDa
• GenBank Accession Number: BC105796
• Gene Symbol: SAA1
• Gene ID (NCBI): 6288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serum amyloid A1(SAA1) is a member of the serum amyloid A family of apolipoproteins. SAA1 is an acute phase apolipoprotein reactant produced mainly by hepatocytes and under regulation of inflammatory cytokines. Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA1 protein. Elevated serum SAA1 protein levels may be associated with lung cancer(PMID: 1463770). This antibody can recognize SAA1 and SAA2.

Publications

Species	Application	Title
human	WB,IHC	Mol Oncol; Serum amyloid A1 in combination with integrin αVβ3 increases glioblastoma cells mobility and progression.; Authors - Ching-Yu Lin ; KD Validated
human	IHC	J Transl Med; Plasma proteomic analysis of autoimmune hepatitis in an improved AIH mouse model.; Authors - Han Wang

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