S1PR5/EDG8 Polyclonal antibody

S1PR5/EDG8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13874-1-AP

Synonyms

Edg 8, EDG8, S1P receptor 5, S1P receptor Edg 8, S1P5, S1PR5, S1PR5/EDG8, SPPR 1, SPPR 2



Tested Applications

Positive WB detected inmouse brain tissue, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13874-1-AP targets S1PR5/EDG8 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen S1PR5/EDG8 fusion protein Ag4928
Full Name sphingosine-1-phosphate receptor 5
Calculated Molecular Weight 398 aa, 42 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC034703
Gene Symbol S1PR5
Gene ID (NCBI) 53637
RRIDAB_2182638
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sphingosine 1-phosphate (S1P) is a biologically active lysophospholipid that elicits diverse physiological effects on most types of cells and tissues via five G protein-coupled receptors, S1PR1 to S1PR5. S1PRs differ in their tissue expression and cell lineage: S1PR1-3 are expressed throughout the immune, central nervous system (CNS), and cardiovascular system; S1PR4 is primarily expressed in lymphoid tissue; and S1PR5 is a predominant receptor in CNS white matter, spleen and natural killer cells (PMID: 21707490). S1PR5 (also known as EDG8) has been involved in the trafficking of NK cells and monocytes (PMID: 21911833; 23519784).

Protocols

Product Specific Protocols
WB protocol for S1PR5/EDG8 antibody 13874-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Sphingosine 1-phosphate (S1P) reduces hepatocyte growth factor-induced migration of hepatocellular carcinoma cells via S1P receptor 2.

Authors - Rie Matsushima-Nishiwaki
mouseWB

Drug Des Devel Ther

Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence.

Authors - Dianna Liu