S1PR5/EDG8 Polyclonal antibody

S1PR5/EDG8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13874-1-AP

Synonyms

Edg 8, EDG8, S1P receptor 5, S1P receptor Edg 8, S1P5, S1PR5, S1PR5/EDG8, SPPR 1, SPPR 2

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Validation Data Gallery

• 

  WB analysis of mouse brain using 13874-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13874-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13874-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13874-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13874-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

13874-1-AP targets S1PR5/EDG8 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S1PR5/EDG8 fusion protein Ag4928
• Full Name: sphingosine-1-phosphate receptor 5
• Calculated Molecular Weight: 398 aa, 42 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC034703
• Gene Symbol: S1PR5
• Gene ID (NCBI): 53637
• RRID: AB_2182638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sphingosine 1-phosphate (S1P) is a biologically active lysophospholipid that elicits diverse physiological effects on most types of cells and tissues via five G protein-coupled receptors, S1PR1 to S1PR5. S1PRs differ in their tissue expression and cell lineage: S1PR1-3 are expressed throughout the immune, central nervous system (CNS), and cardiovascular system; S1PR4 is primarily expressed in lymphoid tissue; and S1PR5 is a predominant receptor in CNS white matter, spleen and natural killer cells (PMID: 21707490). S1PR5 (also known as EDG8) has been involved in the trafficking of NK cells and monocytes (PMID: 21911833; 23519784).

Publications

Species	Application	Title
human	WB	PLoS One; Sphingosine 1-phosphate (S1P) reduces hepatocyte growth factor-induced migration of hepatocellular carcinoma cells via S1P receptor 2.; Authors - Rie Matsushima-Nishiwaki
mouse	WB	Drug Des Devel Ther; Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence.; Authors - Dianna Liu

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