S1PR1 Recombinant antibody, PBS Only (Capture/Detector)

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  Sandwich ELISA standard curve of MP01781-3

  Sandwich ELISA standard curve of MP01781-3, Human S1PR1/EDG1 Recombinant Matched Antibody Pair - PBS only. 85059-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag28083. 85059-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP01781-3

  The mean S1PR1/EDG1 concentration was determined to be 3.08 ng/mL in SH-SY5Y cell extract based on a 3.2 mg/mL extract load and 2.11 ng/mL in MDA-MB-231 cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP01781-1

  Cytometric bead array standard curve of MP01781-1, S1PR1/EDG1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85059-2-PBS. Detection antibody: 85059-1-PBS. Standard: Ag28083. Range: 0.313-40 ng/mL.

S1PR1 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85059-2-PBS

Clone No.242592D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

EDG 1, ECGF1, D1S3362, CHEDG1, CD363

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Product Information

85059-2-PBS targets S1PR1 as part of a matched antibody pair:

MP01781-1: 85059-2-PBS capture and 85059-1-PBS detection (validated in Cytometric bead array)

MP01781-3: 85059-4-PBS capture and 85059-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: S1PR1 fusion protein Ag28083
• Full Name: sphingosine-1-phosphate receptor 1
• Calculated Molecular Weight: 43 kDa
• GenBank Accession Number: BC018650
• Gene Symbol: S1PR1
• Gene ID (NCBI): 1901
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21453
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.