S100G/Calbindin-D9k Polyclonal antibody

S100G/Calbindin-D9k Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 18340-1-AP

Synonyms

CABP, CABP1, CABP9K, CALB3, Calbindin D9k, Protein S100 G, S100 calcium binding protein G, S100D, S100G, S100G/Calbindin-D9k

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Validation Data Gallery

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  IHC staining of mouse small intestine using 18340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18340-1-AP (S100G/Calbindin-D9k antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 18340-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 18340-1-AP (S100G/Calbindin-D9k antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18340-1-AP targets S100G/Calbindin-D9k in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100G/Calbindin-D9k fusion protein Ag13109
• Full Name: S100 calcium binding protein G
• Calculated Molecular Weight: 79 aa, 9 kDa
• GenBank Accession Number: BC112174
• Gene Symbol: S100G
• Gene ID (NCBI): 795
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100G also named calbindin D9K, is a vitamin D-dependent calcium-binding protein. This cytosolic protein belongs to a family of calcium-binding proteins that includes calmodulin, parvalbumin, troponin C, and S100 protein. In the intestine, the protein is vitamin D-dependent and its expression correlates with calcium transport activity. The protein may increase Ca2+ absorption by buffering Ca2+ in the cytoplasm and increase ATP-dependent Ca2+ transport in duodenal basolateral membrane vesicles.