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S100A9 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat No : MP00569-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00569-4, Human S100A9 Recombinant Matched Antibody Pair - PBS only. 83578-8-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25764. 83578-6-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

    Sandwich ELISA standard curve of MP00569-4

    Sandwich ELISA standard curve of MP00569-4, Human S100A9 Recombinant Matched Antibody Pair - PBS only. 83578-8-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag25764. 83578-6-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Serum of seven individual healthy human donors was measured. The S100A9 concentration of detected samples was determined to be 3,005.7 pg/mL with a range of 818.9-12,431.7 pg/mL.

    Sample test of MP00569-4

    Serum of seven individual healthy human donors was measured. The S100A9 concentration of detected samples was determined to be 3,005.7 pg/mL with a range of 818.9-12,431.7 pg/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00569-4 targets S100A9 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: S100A9 Recombinant antibody, PBS Only (Capture) 83578-8-PBS (240539A6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: S100A9 Recombinant antibody, PBS Only (Detector) 83578-6-PBS (240539F4). 100 μg. Concentration 1 mgl/ml.

Alternative S100A9 matched antibody pairs: MP00569-1, MP00569-2, MP00569-3

Matched pair protein standard: Ag25764

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83578-8-PBS
Tested Reactivity human
Immunogen S100A9 fusion protein Ag25764
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name S100 calcium binding protein A9
Synonyms
GenBank accession numberBC047681
Gene symbol S100A9
UniprotP06702
Gene ID (NCBI) 6280
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83578-6-PBS
Tested Reactivity human
Immunogen S100A9 fusion protein Ag25764
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name S100 calcium binding protein A9
Synonyms CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB
GenBank accession numberBC047681
Gene symbol S100A9
UniprotP06702
Gene ID (NCBI) 6280
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.