S100A5 Polyclonal antibody

S100A5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17924-1-AP

Synonyms

S100D, S100 calcium-binding protein A5, Protein S-100D, Protein S100-A5, Protein S100 A5

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Validation Data Gallery

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  WB analysis of HepG2 using 17924-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 17924-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17924-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 17924-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human meningioma using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human meningioma using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human meningioma using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human meningioma using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human brain using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17924-1-AP (S100A5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17924-1-AP (S100A5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17924-1-AP

  Immunofluorescent analysis of HepG2 cells using 17924-1-AP (S100A5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, human kidney tissue, human liver tissue, SH-SY5Y cells
• Positive IHC detected in: human meningioma tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

17924-1-AP targets S100A5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100A5 fusion protein Ag12342
• Full Name: S100 calcium binding protein A5
• Calculated Molecular Weight: 110 aa, 13 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC093955
• Gene Symbol: S100A5
• Gene ID (NCBI): 6276
• RRID: AB_2183794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC,IF	Adv Sci (Weinh); S100A5 Attenuates Efficiency of Anti-PD-L1/PD-1 Immunotherapy by Inhibiting CD8+ T Cell-Mediated Anti-Cancer Immunity in Bladder Carcinoma; Authors - Huihuang Li
mouse	IHC	Mol Cell Proteomics; The Membrane Proteome of Sensory Cilia to the Depth of Olfactory Receptors.; Authors - Katja Kuhlmann