S100A4 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

S100A4 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G11B4

Cat no : 66489-1-PBS

Synonyms

18A2, 42A, Calvasculin, CAPL, Fibroblast-specific protein 1, FSP1, FSP-1, Metastasin, MTS1, P9KA, PEL98, Protein Mts1, Protein S100 A4, S100A4

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Validation Data Gallery

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  WB analysis using 66489-1-Ig (same clone as 66489-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66489-1-Ig (same clone as 66489-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66489-1-Ig (same clone as 66489-1-PBS)

  WB result of S100A4 antibody (66489-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A4 transfected HeLa cells. This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66489-1-Ig (same clone as 66489-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 66489-1-Ig (same clone as 66489-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 66489-1-Ig (same clone as 66489-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66489-1-Ig (S100A4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

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  IHC staining of K-562 using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded k562 slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66489-1-Ig (S100A4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66489-1-Ig (S100A4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66489-1-Ig (same clone as 66489-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S100A4 antibody (66489-1-Ig, Clone: 2G11B4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66489-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66489-1-PBS targets S100A4 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: S100A4 fusion protein Ag9019
• Full Name: S100 calcium binding protein A4
• Calculated Molecular Weight: 101 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC016300
• Gene Symbol: S100A4
• Gene ID (NCBI): 6275
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.