S100A14 Polyclonal antibody

• Featured Product
• KD/KO Validated

S100A14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

28

Cat no : 10489-1-AP

Synonyms

S114, S100A15, S100 calcium-binding protein A14, Protein S100-A14, Protein S100 A14

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Validation Data Gallery

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  WB analysis of MCF-7 using 10489-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10489-1-AP (S100A14 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10489-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10489-1-AP (S100A14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10489-1-AP

  IP result of anti-S100A14 (IP:10489-1-AP, 4ug; Detection:10489-1-AP 1:1000) with MCF-7 cells lysate 760 ug.

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  IHC staining of human colon cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 10489-1-AP (S100A14 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 10489-1-AP (S100A14 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10489-1-AP (S100A14 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10489-1-AP (S100A14 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10489-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10489-1-AP (S100A14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10489-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using S100A14 antibody (10489-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, rat stomach tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human colon cancer tissue, human skin tissue, human ovary tumor tissue, human breast cancer tissue, human bowen disease, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IHC: See 18 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

10489-1-AP targets S100A14 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100A14 fusion protein Ag0757
• Full Name: S100 calcium binding protein A14
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 10-12 kDa
• GenBank Accession Number: BC005019
• Gene Symbol: S100A14
• Gene ID (NCBI): 57402
• RRID: AB_2183628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).

Publications

Species	Application	Title
human	WB,IHC	Oncotarget; The role of S100A14 in epithelial ovarian tumors.; Authors - Hanbyoul Cho
human	WB, IHC	Oncogene; S100A14 suppresses metastasis of nasopharyngeal carcinoma by inhibition of NF-kB signaling through degradation of IRAK1.; Authors - Dong-Fang Meng
human	IF	Am J Respir Cell Mol Biol; Transcriptomics Analysis Identifies the Decline in the AT2 Stem Cell Niche in Aged Human Lungs; Authors - Xue Liu
human	WB, IHC	Int J Cancer; S100A14 is a novel independent prognostic biomarker in the triple-negative breast cancer subtype.; Authors - Sidse Ehmsen
human	WB,IHC,IF	Cell Death Dis; Calcium-binding protein S100A14 induces differentiation and suppresses metastasis in gastric cancer.; Authors - Min Zhu
human	IHC,WB	Am J Cancer Res; A new 7-gene survival score assay for pancreatic cancer patient prognosis prediction.; Authors - Lisi Luo