S100A11 Polyclonal antibody

• Featured Product
• KD/KO Validated

S100A11 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

50

Cat no : 10237-1-AP

Synonyms

Protein S100-A11, Protein S100 C, Protein S100 A11, MLN70, MLN 70

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Validation Data Gallery

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  WB analysis using 10237-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 cells using 10237-1-AP

  WB result of S100A11 antibody (10237-1-AP, 1:4000) with si-control and si-S100A11 transfected PC-3 cells.

• 

  WB analysis using 10237-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10237-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10237-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 10237-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 10237-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 10237-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10237-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10237-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10237-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10237-1-AP (S100A11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 10237-1-AP

  IP result of anti-S100A11 (IP:10237-1-AP, 3ug; Detection:10237-1-AP 1:1000) with PC-3 cells lysate 1000ug.

• 

  IP experiment of DU 145 using 10237-1-AP

  IP result of anti-S100A11 (IP:10237-1-AP, 4ug; Detection:10237-1-AP 1:5000) with DU 145 cells lysate 1280 ug.

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  IHC staining of mouse kidney using 10237-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10237-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10237-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10237-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10237-1-AP (S100A11 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10237-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10237-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10237-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10237-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10237-1-AP (S100A11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10237-1-AP (S100A11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10237-1-AP (S100A11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 10237-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10237-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10237-1-AP (S100A11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 10237-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using S100A11 antibody (10237-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of BxPC-3 using 10237-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using S100A11 antibody (10237-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of BxPC-3 using 10237-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed BxPC-3 cells using S100A11 antibody (10237-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of PC-3 using 10237-1-AP

  1x10^6 PC-3 cells were intracellularly stained with 0.8 ug S100A11 Polyclonal antibody (10237-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: DU 145 cells, HCT 116 cells, BxPC-3 cells, human lung tissue, HEK-293 cells, human heart tissue, COLO 320 cells, HaCaT cells, U2OS cells, SKOV-3 cells
• Positive IP detected in: DU 145 cells, PC-3 cells
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue, human colon cancer tissue, human liver cancer tissue, human lung cancer tissue, human ovary tumor tissue, human thyroid cancer tissue, mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells, BxPC-3 cells
• Positive FC (Intra) detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 37 publications below
• IHC: See 15 publications below
• IF: See 16 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

10237-1-AP targets S100A11 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100A11 fusion protein Ag0343
• Full Name: S100 calcium binding protein A11
• Calculated Molecular Weight: 105 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC001410
• Gene Symbol: S100A11
• Gene ID (NCBI): 6282
• RRID: AB_2183478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A11 (also known as S100C or calziggarin), is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs (PMID: 18694925). S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. First discovered in 1989, S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis, regulation of enzyme activity, cell growth and regulation, apoptosis and inflammation (PMID: 15241500). Chromosomal rearrangements and altered expression of S100A11 have been implicated in tumor metastasis. This S100A11 antibody (10237-1-AP) has also been instrumental in investigations into S100A11's role in the development of brain tumors in tuberous sclerosis complex (TSC) patients (PMID: 20133820).

Publications

Species	Application	Title
human	WB,IF	Nat Commun; S100A11 is required for efficient plasma membrane repair and survival of invasive cancer cells.; Authors - Jyoti K Jaiswal
human	WB	Dev Cell; Annexin A1 Tethers Membrane Contact Sites that Mediate ER to Endosome Cholesterol Transport.; Authors - Emily R Eden
human	IHC	J Pathol; Tissue-based proteomics reveals FXYD3, S100A11 and GSTM3 as novel markers for regional lymph node metastasis in colon cancer.; Authors - Meding Stephan S
human	IF	Mol Cell Proteomics; S100A11 mediates hypoxia-induced mitogenic factor (HIMF)-induced smooth muscle cell migration, vesicular exocytosis, and nuclear activation.; Authors - Fan Chunling C
human	WB, IF, CoIP	Cell Mol Gastroenterol Hepatol; S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis.; Authors - Linqiang Zhang
human, mouse	WB, IHC, IF	J Clin Endocrinol Metab; Down-regulation of S100A11, a calcium-binding protein, in human endometrium may cause reproductive failure.; Authors - Liu Xin-Mei XM

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