S100A1 Monoclonal antibody

S100A1 Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A8C1

Cat no : 67237-1-Ig

Synonyms

Protein S100 A1, S 100 protein alpha chain, S 100 protein subunit alpha, S100, S100 alpha, S100A, S100A1

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Validation Data Gallery

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  IHC staining of human tonsillitis using 67237-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67237-1-Ig (S100A1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67237-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67237-1-Ig (S100A1 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67237-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67237-1-Ig (S100A1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67237-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67237-1-Ig (S100A1 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:16000-1:64000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67237-1-Ig targets S100A1 in IHC, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: S100A1 fusion protein Ag19544
• Full Name: S100 calcium binding protein A1
• Calculated Molecular Weight: 94 aa, 11 kDa
• GenBank Accession Number: BC014392
• Gene Symbol: S100A1
• Gene ID (NCBI): 6271
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.