S100B Recombinant antibody

S100B Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E2

Cat no : 82271-8-RR

Synonyms

S100 beta, S-100 protein beta chain, S100 calcium-binding protein B, Protein S100-B, Protein S100 B

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Validation Data Gallery

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  WB analysis using 82271-8-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82271-8-RR (S100B antibody) at dilution of 1:5150 incubated at room temperature for 1.5 hours.

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  FC experiment of A375 using 82271-8-RR

  1x10^6 A375 cells were intracellularly stained with 0.25 ug S100B Recombinant antibody (82271-8-RR, Clone:1E2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82271-8-RR

  Biolayer interferometry (BLl) kinetic assays of 82271-8-RR against Human S100 Beta were performed. The affinity constant is 38.5 pM.

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Tested Applications

• Positive WB detected in: C6 cells, A375 cells, rat brain tissue
• Positive FC (Intra) detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10300
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82271-8-RR targets S100B in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: S100B fusion protein Ag7440
• Full Name: S100 calcium binding protein B
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC001766
• Gene Symbol: S100 Beta
• Gene ID (NCBI): 6285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. In the nervous system, S100B is constitutively released by astrocytes into the extracellular space and at nanomolar concentrations, it can promote neurite outgrowth and protect neurons against oxidative stress. Within the central nervous system (CNS), S100B is thought to be a marker for astroglial activation, linking astrocyte dysfunction to schizophrenia. In addition to astrocytes, S100B is released from many cell types, such as, adipocytes, chondrocytes, cardiomyocytes and lymphocytes. Serum S100B represents a new biomarker for mood disorders.