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Recombinant NTRK3 (454-825) protein

Expressed In

Baculovirus

Protein Species

Human

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Cat No : 81383

Validation Data Gallery

  • Recombinant NTRK3 (454-825) protein 10% SDS-PAGE with Coomassie staining MW: 43.5 kDa Purity: >90%

    Recombinant NTRK3 (454-825) protein 10% SDS-PAGE with Coomassie staining MW: 43.5 kDa Purity: >90%

  • HTRF assay for NTRK3 (454-825) activity 1 uM TK substrate was incubated with different concentrations of NTRK3 (454-825) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

    HTRF assay for NTRK3 (454-825) activity 1 uM TK substrate was incubated with different concentrations of NTRK3 (454-825) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

  • HTRF assay for NTRK3 (454-825) activity 1 uM TK substrate was incubated with different concentrations of NTRK3 (454-825) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

    HTRF assay for NTRK3 (454-825) activity 1 uM TK substrate was incubated with different concentrations of NTRK3 (454-825) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

Product Information

Expressed In Baculovirus
Protein Species Human

Contents

Recombinant NTRK3 (454-825) protein is supplied in 25 mM HEPESNaOH pH 7.5, 300 mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.

Background

NTRK3 (Neurotrophic Receptor Tyrosine Kinase 3) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers.

Application Notes

This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant NTRK3 (454-825) protein that includes amino acids 454-825 of human NTRK3 protein (accession number NP_001362740.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 43.5 kDa.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.