Recombinant FBXL10 / KDM2B protein
Expressed In
Baculovirus
Protein Species
Human
Cat No : 31455
Validation Data Gallery
Product Information
| Expressed In | Baculovirus |
| Protein Species | Human |
Contents
Full length recombinant FBXL10 / KDM2B protein expressed in Sf9 cells and is supplied in 25 mM HEPES-NaOH, pH 7.5, 300 mM NaCl, 5% glycerol and 0.2 mg/ml 3x Flag peptide.
Background
KDM2B (lysine (K)-specific demethylase 2B), also known as FBXL10 (F-box and leucine-rich repeat protein 10) is a histone demethylase that preferentially demethylates trimethylated lysine 4 (K4me3) and dimethylated lysine 36 (K36me2) of histone H3. KDM2B displays weak or no activity for mono- and trimethylated H3K36. KDM2B preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which results in inhibition of cell growth and proliferation. KDM2B may also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Application Notes
Recombinant FBXL10 / KDM2B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP, 300 nM Recombinant FBXL10 / KDM2B protein, and 3.3 μM H3K36me2 peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
Protein Details
Recombinant FBXL10 / KDM2B (accession number NP_115979.3) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 155.9 kDa. The recombinant protein is >75% pure by SDS-PAGE.
Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.