Rat IL-1 beta ELISA Kit

Sensitivity

12.5 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Rat

Cat no : KE20005

Synonyms

IL 1, IL 1 BETA, IL 1BETA, IL1, IL-1, IL1 BETA, IL-1 beta, Il1b, IL-1B, IL1BETA, IL1β, interleukin 1 beta, Interleukin-1 beta



Product Information

KE20005 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL1-beta ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1 beta. The assay recognizes rat IL1-beta. An antibody specific for IL1-beta has been pre-coated onto the microwells. The IL-1 beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-1 beta is added to detect the captured IL-1 beta protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Rat IL-1 beta ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 12.5 pg/mL
Range 31.25-2000 pg/mL
Reactivity Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 24494

Recovery

Sample TypeAverageRange
Rat Serum84%77%-97%
Cell culture supernatants107%93%-118%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12076.43.04.0
220243.010.24.2
320849.230.14.4

InterAssay

Samplenmean ( pg/mL)SDCV%
12450.14.28.3
224190.37.33.8
324684.717.42.5

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL1 gene family encodes three proteins: IL1 -alpha IL1- beta and their naturally occurring inhibitor IL1-RN. Interleukin 1 beta(IL1- beta), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL1-beta is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL1-beta is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms IL 1, IL 1 BETA, IL 1BETA, IL1, IL-1, IL1 BETA, IL-1 beta, Il1b, IL-1B, IL1BETA, IL1β, interleukin 1 beta, Interleukin-1 beta

Publications

SpeciesSample TypeTitle
ratplasma

Nat Commun

Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.

Authors - Ziwen Wang
ratcell culture supernatants

Oxid Med Cell Longev

Metformin Activates the Protective Effects of the AMPK Pathway in Acute Lung Injury Caused by Paraquat Poisoning.

Authors - Liaozhang Wu
ratcell culture supernatants

Oxid Med Cell Longev

Dexmedetomidine Alleviates Hypoxia-Induced Synaptic Loss and Cognitive Impairment via Inhibition of Microglial NOX2 Activation in the Hippocampus of Neonatal Rats.

Authors - Xiaohui Chen
rattissue homogenates

Br J Pharmacol

Basolateral amygdala astrocytes modulate diabetic neuropathic pain and may be a potential therapeutic target for koumine

Authors - Jing-Shan Lu
humancell culture supernatants

Nanotoxicology

Role of inflammation in the malignant transformation of pleural mesothelial cells induced by multi-walled carbon nanotubes.

Authors - Xiaopei Huang
serum

Cell Prolif

Kcnh2 mediates FAK/AKT-FOXO3A pathway to attenuate sepsis-induced cardiac dysfunction.

Authors - Zhigang Li