Rat Adiponectin ELISA Kit
Cat no : KE20016
Synonyms
Acdc, Acrp30, Adipoq
Validation Data Gallery
Product Information
KE20016 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Adiponectin ELISA kit is to be used to detect and quantify protein levels of endogenous Adiponectin. The assay recognizes rat Adiponectin. An antibody specific for Adiponectin has been pre-coated onto the microwells. The Adiponectin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Adiponectin is added to detect the captured Adiponectin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat Adiponectin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.0 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 246253 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 107% | 81% - 121% |
| Cell culture supernatants | 89% | 77%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,171.0 | 43.4 | 3.7 |
| 2 | 20 | 290.0 | 8.9 | 3.1 |
| 3 | 20 | 68.5 | 2.6 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,194.9 | 63.9 | 5.4 |
| 2 | 24 | 301.8 | 14.8 | 4.9 |
| 3 | 24 | 71.3 | 3.1 | 4.3 |
Background Information
Adiponectin, also known as Acrp30, AdipoQ, and GBP28, is an adipocyte-secreted protein that circulates in high concentrations in the serum and acts to increase insulin sensitivity. Adiponectin modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. The serum concentration of adiponectin decreases in obese patients, opposite to other adipocytokines. In recent studies, serum adiponectin level progressively decreased in patients according to the severity of obesity, insulin resistance, diabetes mellitus and cardiovascular diseases. Low serum adiponectin level may be a predictive factor for type 2 diabetes mellitus (T2DM) and other cardiovascular diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Acdc, Acrp30, Adipoq |