CoraLite® Plus 647-conjugated RYR2 Polyclonal antibody

RYR2 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-27587

Synonyms

ARVC2, ARVD2, ryanodine receptor 2 (cardiac), RYR2, VTSIP

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Validation Data Gallery

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  IF Staining of mouse heart using CL647-27587

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 RYR2 antibody (CL647-27587) at dilution of 1:200.

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  IF Staining of mouse heart using CL647-27587

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 647 RYR2 antibody (CL647-27587) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse heart tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-27587 targets RYR2 in IF applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RYR2 fusion protein Ag26296
• Full Name: ryanodine receptor 2 (cardiac)
• Calculated Molecular Weight: 565 kDa
• GenBank Accession Number: NM_001035
• Gene Symbol: RYR2
• Gene ID (NCBI): 6262
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RYR2 belongs to the ryanodine receptor family. RYR2 provides communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules. Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia type 2 (ARVD2) which known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). Defects in RYR2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) which known as stress-induced polymorphic ventricular tachycardia (VTSIP).