RYK Polyclonal antibody

• Featured Product
• KD/KO Validated

RYK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22138-1-AP

Synonyms

Tyrosine protein kinase RYK, RYK1, JTK5A, JTK5, EC:2.7.10.1

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Validation Data Gallery

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  WB analysis of A549 using 22138-1-AP

  WB result of RYK antibody (22138-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RYK transfected A549 cells.

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  WB analysis of Jurkat using 22138-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22138-1-AP (RYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22138-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22138-1-AP (RYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 22138-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22138-1-AP (RYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 22138-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22138-1-AP (RYK antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 22138-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22138-1-AP (RYK antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 22138-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 22138-1-AP (RYK antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 22138-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 22138-1-AP (RYK antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of SH-SY5Y using 22138-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 22138-1-AP (RYK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, A549 cells, mouse brain tissue, SH-SY5Y cells
• Positive IHC detected in: human kidney tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

22138-1-AP targets RYK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RYK fusion protein Ag17540
• Full Name: RYK receptor-like tyrosine kinase
• Calculated Molecular Weight: 610 aa, 68 kDa
• Observed Molecular Weight: 67-75 kDa
• GenBank Accession Number: BC153090
• Gene Symbol: RYK
• Gene ID (NCBI): 6259
• RRID: AB_11232602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tyrosine-protein kinase RYK may be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A, and WNT5A. It is involved in neuron differentiation, axon guidance, corpus callosum establishment, and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region, allowing the C-terminal intracellular product to translocate from the cytoplasm to the nucleus, which plays a crucial role in neuronal development.

Publications

Species	Application	Title
mouse	WB	J Neuroinflammation; Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model; Authors - Yitian Lu
human	WB	Cell Mol Biol Lett; Downregulation of exosomal miR-7-5p promotes breast cancer migration and invasion by targeting RYK and participating in the atypical WNT signalling pathway; Authors - Zhaoyi Liang
human	WB	Pharmaceuticals (Basel); RYK Gene Expression Associated with Drug Response Variation of Temozolomide and Clinical Outcomes in Glioma Patients; Authors - Ricardo D Gonzalez