RXRG Polyclonal antibody

RXRG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, PLA, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11129-1-AP

Synonyms

NR2B3, Retinoid X receptor gamma, retinoid X receptor, gamma, RXRC, RXRG

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Validation Data Gallery

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  WB analysis of mouse heart using 11129-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse ovary using 11129-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11129-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat eye using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat eye using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, Jurkat cells, mouse ovary tissue
• Positive IHC detected in: rat eye tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

11129-1-AP targets RXRG in WB, IF, PLA, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RXRG fusion protein Ag1611
• Full Name: retinoid X receptor, gamma
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51-55 kDa
• GenBank Accession Number: BC012063
• Gene Symbol: RXRG
• Gene ID (NCBI): 6258
• RRID: AB_2182635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RXRG, also named as, Retinoic acid receptor RXR-gamma, is a 463 amino acid protein, which localize in the nucleus and may form homodimer. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5.

Publications

Species	Application	Title
mouse	PLA	Aging (Albany NY); Cholecalciferol (vitamin D3) has a direct protective activity against interleukin 6-induced atrophy in C2C12 myotubes.; Authors - Maraiza Alves Teixeira
rat	IF	Am J Physiol Lung Cell Mol Physiol; Inhaled vitamin A is more effective than intramuscular dosing in mitigating hyperoxia-induced lung injury in a neonatal rat model of bronchopulmonary dysplasia.; Authors - Craig A Gelfand
human	WB	Cells; EGFR/ErbB Inhibition Promotes OPC Maturation up to Axon Engagement by Co-Regulating PIP2 and MBP.; Authors - Emanuela Nocita