RUVBL1 Monoclonal antibody

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  WB analysis using 67750-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67750-1-Ig (RUVBL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67750-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67750-1-Ig (RUVBL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

RUVBL1 Monoclonal Antibody for WB, ELISA

Cat No. 67750-1-Ig

Clone No.1F2D9

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, ELISA

Synonyms

49 kDa TBP interacting protein, ECP 54, ECP54, INO80 complex subunit H, INO80H, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, RuvB like 1, RuvB like 1 (E. coli), RUVBL1, RVB1, TAP54 alpha, TIH1, TIP49, TIP49A

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Tested Applications

• Positive WB detected in: U2OS cells, HeLa cells, HT-1080 cells, HCT 116 cells, K-562 cells, A549 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67750-1-Ig targets RUVBL1 in WB, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RUVBL1 fusion protein Ag16688
• Full Name: RuvB-like 1 (E. coli)
• Calculated Molecular Weight: 456 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC002993
• Gene Symbol: RUVBL1
• Gene ID (NCBI): 8607
• RRID: AB_2918519
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9Y265
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198].